Les Houches

Thematic school 2021

All-optical interrogation of neuronal networks in vivo

In Les Houche, 25-30 april 2021

Thanks to the development of optogenetics, activity of neural networks can be recorded and modulated using optical methods. Thereby, these methods have become major tools for studying the neural mechanisms underlying perception, memory and behavior in animal models.

In this school, we will describe the different fluorescence microscopy techniques that have been developed and used to record neuronal activity in vivo, as well as the methods that enable modulating activity according to precise spatio-temporal patterns. We will first discuss the theoretical bases of these methods, and then we will present recent advances that have improved their speed, depth of penetration, field of view (2D and 3D), and applicability to the awake animal, both in head-fixed and unconstrained configurations. We will also give an overview of the palette of optogenetic tools available and the associated targeted labeling technologies. Finally, we will discuss the methods for analysis of raw functional signals, showing the wealth of information that these experiments can provide.

The school is designed for students and researchers in neurophysiology using optical methods and for physicists participating in their development.

Ecoles thématiques 2021

All-optical interrogation of neuronal networks in vivo

Enregistrement et manipulation de l’activité des réseaux de neurones in vivo avec la lumière

Les Houches, 25-30 avril 2021

Grâce au développement de l'optogénétique, les méthodes optiques permettent d’enregistrer et de moduler l'activité de réseaux neuronaux. Elles sont devenues des outils majeurs pour étudier les mécanismes neuronaux sous-tendant la perception, la mémoire et les comportements chez des modèles animaux.

Dans cette école, nous décrirons les différentes techniques de microscopie de fluorescence donnant accès à l’enregistrement de l’activité neuronale in vivo ainsi que les méthodes permettant de moduler l’activité selon des motifs spatio-temporels précis. Nous aborderons leurs bases théoriques puis nous présenterons les progrès récents qui ont permis d’améliorer leur vitesse, profondeur de pénétration, champ (2D et 3D), et applicabilité à l’animal éveillé tête-fixée puis non contraint. Nous décrirons également la variété des outils optogénétiques disponibles et les technologies de marquage ciblé associées. Enfin, nous aborderons les méthodes d’analyse des signaux fonctionnels bruts, montrant la richesse des informations que ces expériences peuvent apporter.  

L’école s’adresse à des étudiants et chercheurs en neurophysiologie utilisateurs de méthodes optiques et à des physiciens participant à leur développement.

 

Ecoles thématiques 2021

All-optical interrogation of neuronal networks in vivo

Les Houches, 25-30 avril 2021

 

Organisateurs

Cathie Ventalon (IBENS), Laurent Bourdieu (IBENS)

 

Agenda

Campagne de soumission : 26 novembre 2020 - 1 février 2021
Réponse aux candidats : 20 février 2021
Campagne d'inscription : 20 février - 20 mars 2021
Paiement ou bon de commande impérativement avant le 31 mars 2021

 

Schedule

Submission campaign : November 26th, 2020 - February 1st, 2021
Answer to candidates : February 20th, 2021
Registration campaign : February 20th - March 20th, 2021
Payment or order form must be made before 31 March 2021

Sponsoring

2021 SFO THEMATIC SCHOOL - COMMERCIAL SPONSORSHIP OFFER | VISIBILITY OF YOUR COMPANY

- logo of your company on the site and the program with link url
- documentation in the pupils' locker (document of 2 pages max, A4 size max)
600 € TTC [500,00 € HT + 100,00 € (€ VAT)]

2021 SFO THEMATIC SCHOOL - COMMERCIAL SPONSORSHIP OFFER | WITH DEMONSTRATION

- demonstration on tabletop (hall or dedicated room ) On a specific time slot and included in the program on Wednesday April 28nd
- 1 person presents for free, meal offered, the day of the demonstration (*)
- logo of your company on the site and the program with link url
- documentation in the pupils' pocket (document of 2 pages max, A4 size max)
(*) The travel and the stay of this person must be planned by your care, off-site of the School, and not supported by this commercial offer
1 200,00 € TTC [1 000,00 € HT + 200,00 € (€ VAT)]

2021 SFO THEMATIC SCHOOL - ONE PERSON EXTRA

- 1 person presents, meal offered, the day of the demonstration (*)
(*) The travel and the stay of this person must be planned by your care, off-site of the School, and not supported by this commercial offer
102,00 € TTC [85,00 € HT + 17,00 € (€ VAT)]

 

ONLINE BOOKING FROM HERE

 

les Houches

Les Houches is a village located in Chamonix valley, in the French Alps.

Established in 1951, the Physics School is situated at 1150 m above sea level in natural surroundings, with breathtaking views on the Mont-Blanc mountain range. Les Houches Physics School is funded by Université Grenoble Alpes, CNRS, Grenoble-INP, ENS Lyon, and CEA. Ecole de Physique des Houches, 149 Chemin de la Côte, F-74310 Les Houches, France.

Practical information

Map

https://houches.univ-grenoble-alpes.fr/

Les houches

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